Luminex IS Version 2.3 User Manual download pdf (Page 35)

xMAP Technology Luminex 2.3 Software

PN 89-00002-00-110 Rev. B 25

Figure 19 Competitive Assay

The standards tab displays an Expected Concentration column for

standard and control samples. The Standards Expected Concentration

column allows users to edit the standard concentrations. You can edit

controls; however, you must select the control name or Expected

Concentration box for that control, then click Change Lot.

When editing standard and control lot values the system may prompt

you for a new number. If this is the first batch you analyze using this

lot number, the system allows editing without requiring a new lot

name. However, if you have analyzed a previous batch using this lot,

the system will require that you rename the lot if edited or modified.

Background samples are commonly used in the assay process.

Although recommended, the discretion on whether to use or not is

left to the assay or kit developer. If background samples are included

they are defined in the assay template.

Background samples are samples with no active test substance. The

system uses background samples to remove assay background noise

from the sample results. Typical background samples contain

coupled beads,

assay buffer, detection reagents, and reporter

fluorophore. Background samples do not contain target analytes.

If the background sample is designated accordingly, the system

subtracts the background sample’s reported fluorescence from all

other samples in a batch to report net MFI. If a batch contains more

than one background sample, the fluorescence values of the two

background samples are averaged together and subtracted from the

other samples to obtain a net fluorescence intensity.

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Luminex IS Version 2.3 User Manual Page 35