Luminex 100 User Manual Version 1.7 User Manual

Luminex 100™ User Manual Version 1.7 Lum/nex ®

vi PN 89-00002-00-063 Rev. ALuminex 100 User Manual Version 1.7 xMAP Technology

Luminex 100 User Manual Version 1.7 xMAP Technology8 - 6 PN 89-00002-00-063 Rev. ACalibration ProblemsSample arm does not go down smoothly. The 96-wel

xMAP Technology TroubleshootingPN 89-00002-00-063 Rev. A 8 - 7The wrong wells are selected in the XY tab in the Luminex Data Collector software.On the

Luminex 100 User Manual Version 1.7 xMAP Technology8 - 8 PN 89-00002-00-063 Rev. AAcquisition ProblemsThe calibration microspheres are not fully suspe

xMAP Technology TroubleshootingPN 89-00002-00-063 Rev. A 8 - 9The sheath or waste lines are not fully connected.Check to make sure all tubes are tight

Luminex 100 User Manual Version 1.7 xMAP Technology8 - 10 PN 89-00002-00-063 Rev. ABead Detail Irregularities You can diagnose many problems by using

xMAP Technology TroubleshootingPN 89-00002-00-063 Rev. A 8 - 11Figure 8-2. Normal HistogramSymptom Possible Problem SolutionCalibration microspheres

Luminex 100 User Manual Version 1.7 xMAP Technology8 - 12 PN 89-00002-00-063 Rev. AError MessagesMicrospheres appear as a long diagonal lineThe xMAP m

xMAP Technology TroubleshootingPN 89-00002-00-063 Rev. A 8 - 13“...an increased usage of power to the green laser.”A warmup error occurred. If the mes

Luminex 100 User Manual Version 1.7 xMAP Technology8 - 14 PN 89-00002-00-063 Rev. APrinting ErrorsVerificationPerform the verification procedure follo

xMAP Technology TroubleshootingPN 89-00002-00-063 Rev. A 8 - 15the sample probe is properly aligned for use with the plate chosen for use. 7. Click Op

PN 89-00002-00-063 Rev. A 1 - 1IntroductionThe Luminex® 100 system is a compact analysis unit consisting of a Luminex 100 analyzer, the Luminex XY Pla

Luminex 100 User Manual Version 1.7 xMAP Technology8 - 16 PN 89-00002-00-063 Rev. A

PN 89-00002-00-063 Rev. A 9 - 1ProtocolsUpdates or additions to these protocols are posted on the Luminex website at http://luminexcorp.custhelp.com.T

Luminex 100 User Manual Version 1.7 xMAP Technology9 - 2 PN 89-00002-00-063 Rev. A3. Sonicate the vial in a water bath sonicator to disperse the micro

xMAP Technology ProtocolsPN 89-00002-00-063 Rev. A 9 - 3Enumeration of xMAP Microsphere SuspensionsLuminex xMAP microspheres are provided at standard

Luminex 100 User Manual Version 1.7 xMAP Technology9 - 4 PN 89-00002-00-063 Rev. AxMAP Microsphere Stability and StoragexMAP microspheres are light se

xMAP Technology ProtocolsPN 89-00002-00-063 Rev. A 9 - 5Two-Step Carbodiimide Coupling of Protein to xMAP Carboxylated MicrospheresIntroduction Use t

Luminex 100 User Manual Version 1.7 xMAP Technology9 - 6 PN 89-00002-00-063 Rev. APreparation 1. Allow all reagents to warm to room temperature.2. Dil

xMAP Technology ProtocolsPN 89-00002-00-063 Rev. A 9 - 713. Add 500 µL of COUPLING BUFFER to the activated xMAP microspheres and vortex. Centrifuge th

Luminex 100 User Manual Version 1.7 xMAP Technology9 - 8 PN 89-00002-00-063 Rev. AOne-Step Carbodiimide Coupling of Oligonucleotides to xMAP Carboxyla

xMAP Technology ProtocolsPN 89-00002-00-063 Rev. A 9 - 9Procedure 1. Centrifuge the xMAP microsphere stock for 1 minute at a minimum of 8,000 x g.2. D

Luminex 100 User Manual Version 1.7 xMAP Technology1 - 2 PN 89-00002-00-063 Rev. Asignature. In addition, the Luminex 100 analyzer scans each microsph

Luminex 100 User Manual Version 1.7 xMAP Technology9 - 10 PN 89-00002-00-063 Rev. ATechnical notes 1. Oligonucleotides must be synthesized with a 5’ o

xMAP Technology ProtocolsPN 89-00002-00-063 Rev. A 9 - 11• REACTION BUFFER: Phosphate Buffered Saline (pH 7.3 ± 0.1), Bovine Serum Albumin (10 mg/ml)•

Luminex 100 User Manual Version 1.7 xMAP Technology9 - 12 PN 89-00002-00-063 Rev. ATechnical notes 1. The concentration of Biotin-conjugated molecules

PN 89-00002-00-063 Rev. A A - 1Glossaryaggregated microspheresTwo or more xMAP microspheres that have adhered together.Alexa 532 Fluorochrome used for

A - 2 PN 89-00002-00-063 Rev. ALuminex 100 User Manual Version 1.7 xMAP Technologycalibrators xMAP microspheres used to normalize the settings for the

PN 89-00002-00-063 Rev. A A - 3xMAP Technology emission spectrumemission spectrum Wavelength range that an excited fluorochrome emits when its electro

A - 4 PN 89-00002-00-063 Rev. ALuminex 100 User Manual Version 1.7 xMAP TechnologyxMAP microsphere set Luminex multi-analyte microspheres containing a

PN 89-00002-00-063 Rev. A A - 5xMAP Technology reporterreporter A molecule (or combination of molecules) with a specific range of excitation and emiss

A - 6 PN 89-00002-00-063 Rev. ALuminex 100 User Manual Version 1.7 xMAP Technology

PN 89-00002-00-063 Rev. A B - 1Product NumbersNote: These part numbers are subject to change without notification.HardwareBAir filter (accessed throug

xMAP Technology IntroductionPN 89-00002-00-063 Rev. A 1 - 3Excitation The excitation system in the Luminex 100 analyzer uses two solid-state lasers. A

B - 2 PN 89-00002-00-063 Rev. ALuminex 100 User Manual Version 1.7 xMAP TechnologySoftwareReagentsPower cord CN-P012-01 (China)CN-P007-01 (Israel)CN-P

PN 89-00002-00-063 Rev. A B - 3xMAP TechnologyTrainingxMAP development microspheres (carboxylated)1, 4, and 16 mLL100-CDEV1-[size code of 01, 04, or 1

B - 4 PN 89-00002-00-063 Rev. ALuminex 100 User Manual Version 1.7 xMAP Technology

PN 89-00002-00-063 Rev. A C - 1Luminex Sheath Delivery SystemOverview Use this appendix to install, use, and maintain the Luminex Sheath Delivery Syst

Luminex 100 User Manual Version 1.7 xMAP TechnologyC - 2 PN 89-00002-00-063 Rev. AHardware The Luminex SD system includes:• Luminex Sheath Delivery de

xMAP Technology Luminex Sheath Delivery SystemPN 89-00002-00-063 Rev. A C - 3Installation  To install the Luminex SD system:1. Locate the sheath flui

Luminex 100 User Manual Version 1.7 xMAP TechnologyC - 4 PN 89-00002-00-063 Rev. Areceptacle is level with the Luminex 100 analyzer or no more than th

xMAP Technology Luminex Sheath Delivery SystemPN 89-00002-00-063 Rev. A C - 5until the sheath pressure displayed in the System Monitor reads the same

Luminex 100 User Manual Version 1.7 xMAP TechnologyC - 6 PN 89-00002-00-063 Rev. ARegularly, as required. Replace the sheath fluid as required.Weekly.

xMAP Technology Luminex Sheath Delivery SystemPN 89-00002-00-063 Rev. A C - 7Labels The following labels are attached to the back of the Luminex SD.

Luminex 100 User Manual Version 1.7 xMAP Technology1 - 4 PN 89-00002-00-063 Rev. AFluorescence CompensationFluorescent spillover occurs when an emissi

xMAP Technology Luminex Sheath Delivery SystemPN 89-00002-00-063 Rev. A C - 8

PN 89-00002-00-063 Rev. A Index-1Numerics100 region bead map 6-225 region bead map 6-250 region bead map 6-2Aaccess doors 3-6Acquisition tab 6-3additi

Index-2 PN 89-00002-00-063 Rev. ALuminex 100 User Manual Version 1.7 xMAP TechnologyCE mark 2-4centrifugation 9-3changingfuses 7-8sheath filter 7-7ver

PN 89-00002-00-063 Rev. A Index-3xMAP Technology Indexhistogram 6-17buttons 6-19changing X-axis 6-17display 6-10gating 6-18printing 6-28scaling Y-axis

Index-4 PN 89-00002-00-063 Rev. ALuminex 100 User Manual Version 1.7 xMAP TechnologyPhycoerythrin 1-3, A-4port 3-5, 4-3power 3-2, 3-4power cord produc

PN 89-00002-00-063 Rev. A Index-5xMAP Technology Indexspillover, fluorescent 1-4standby 6-23start 6-13, 6-14startup procedure 7-1status 6-22status bar

Index-6 PN 89-00002-00-063 Rev. ALuminex 100 User Manual Version 1.7 xMAP Technology

xMAP Technology IntroductionPN 89-00002-00-063 Rev. A 1 - 5from the main menu bar, from the toolbar, and from menus that appear when you right-click a

Luminex 100 User Manual Version 1.7 xMAP Technology1 - 6 PN 89-00002-00-063 Rev. A

PN 89-00002-00-063 Rev. A 2 - 1SafetyYour safety is important. Please do not perform procedures on your Luminex 100 system that are not specifically c

Luminex 100 User Manual Version 1.7 xMAP Technology2 - 2 PN 89-00002-00-063 Rev. AWarnings and Notes Informational notes and warnings may appear in th

xMAP Technology SafetyPN 89-00002-00-063 Rev. A 2 - 3with the specific safety advisories below, in addition to adhering to standard laboratory safety

© LUMINEX CORPORATION, 1998 - 2004. All rights reserved. No part of this publication may be reproduced, transmitted, transcribed, or translated into a

Luminex 100 User Manual Version 1.7 xMAP Technology2 - 4 PN 89-00002-00-063 Rev. AThis label appears on the back of the Luminex 100 analyzer and the L

xMAP Technology SafetyPN 89-00002-00-063 Rev. A 2 - 5This voltage label displays on the back of the Luminex XYP:Figure 2-6. Luminex XYP Voltage Label

Luminex 100 User Manual Version 1.7 xMAP Technology2 - 6 PN 89-00002-00-063 Rev. AThis label appears on the back panel of the Luminex 100 analyzer:Fig

xMAP Technology SafetyPN 89-00002-00-063 Rev. A 2 - 7Figure 2-10. Avoid Exposure LabelMechanicalBiologicalCaution: Use of controls or adjustments or

Luminex 100 User Manual Version 1.7 xMAP Technology2 - 8 PN 89-00002-00-063 Rev. AHeatBlue Indicator LightThe blue light above the sample arm simply i

PN 89-00002-00-063 Rev. A 3 - 1System OverviewHardware The Luminex 100 Version 1.7 system includes a Luminex 100 Version 1.7 analyzer, the Luminex XYP

Luminex 100 User Manual Version 1.7 xMAP Technology3 - 2 PN 89-00002-00-063 Rev. AIn addition, you can purchase the optional Luminex Sheath Delivery s

xMAP Technology System OverviewPN 89-00002-00-063 Rev. A 3 - 3Laser • Reporter laser: 532 nm, nominal output 10-16.5 mW, maximum 500 mW, frequency-dou

Luminex 100 User Manual Version 1.7 xMAP Technology3 - 4 PN 89-00002-00-063 Rev. ALuminex XYP SpecificationsGeneral • Operating temperature: 15°C to 3

xMAP Technology System OverviewPN 89-00002-00-063 Rev. A 3 - 5Luminex 100 Analyzer OverviewThe Luminex 100 analyzer consists of three subsystems: elec

End-User License Agreement (EULA) for Luminex® SoftwareThis Luminex End-User License Agreement (“EULA”) is a legal agreement between you (either an in

Luminex 100 User Manual Version 1.7 xMAP Technology3 - 6 PN 89-00002-00-063 Rev. AFluidic Sample Arm: Transports the sample from the sample plate to

xMAP Technology System OverviewPN 89-00002-00-063 Rev. A 3 - 7Figure 3-3. Front View of Luminex 100 Analyzer Showing Access DoorsAir filter and acces

Luminex 100 User Manual Version 1.7 xMAP Technology3 - 8 PN 89-00002-00-063 Rev. ASyringe: Delivers sample from the sample plate to the cuvette. It i

xMAP Technology System OverviewPN 89-00002-00-063 Rev. A 3 - 9Figure 3-7. Left Side of Analyzer - Air and Fluid ConnectorsSheath fluid container (not

Luminex 100 User Manual Version 1.7 xMAP Technology3 - 10 PN 89-00002-00-063 Rev. ALuminex XYP Instrument OverviewThe Luminex XYP is a self-contained

PN 89-00002-00-063 Rev. A 4 - 1InstallationLuminex 100 System Installation Ensure that the facility complies with all system and safety requirements.

Luminex 100 User Manual Version 1.7 xMAP Technology4 - 2 PN 89-00002-00-063 Rev. A6. Replace the short sample probe with the long sample probe as foll

xMAP Technology InstallationPN 89-00002-00-063 Rev. A 4 - 3Luminex 100 Software SetupAfter performing the Luminex 100 system assembly, ensure that the

Luminex 100 User Manual Version 1.7 xMAP Technology4 - 4 PN 89-00002-00-063 Rev. AAdjusting the Sample Probe HeightYou must adjust the Sample Probe ve

xMAP Technology InstallationPN 89-00002-00-063 Rev. A 4 - 55. Place the 96-well microtiter plate on the Luminex XYP with position A1 in the top left c

be accessed through use of the SOFTWARE PRODUCT is the property of the respective content owner and may be protected by applicable copyright or other

Luminex 100 User Manual Version 1.7 xMAP Technology4 - 6 PN 89-00002-00-063 Rev. AInstalling Software and FirmwareThe Luminex 100 system arrives prelo

PN 89-00002-00-063 Rev. A 5 - 1 System StartupThis chapter describes how to start up your system. The topics included are:• Luminex Main Window - a to

Luminex 100 User Manual Version 1.7 xMAP Technology5 - 2 PN 89-00002-00-063 Rev. AThe Luminex Main window has six components: Title bar, Menu bar, Too

xMAP Technology System StartupPN 89-00002-00-063 Rev. A 5 - 3 To add a new lot of xMAP classification calibration microspheres:1. Click Calibrate. T

Luminex 100 User Manual Version 1.7 xMAP Technology5 - 4 PN 89-00002-00-063 Rev. AAdd New Reporter Channel Calibration LotsBefore you perform initial

xMAP Technology System StartupPN 89-00002-00-063 Rev. A 5 - 5Calibrate the Luminex 100 AnalyzerIn addition to calibrating the analyzer after installat

Luminex 100 User Manual Version 1.7 xMAP Technology5 - 6 PN 89-00002-00-063 Rev. A7. Fill the reservoir with a solution of 70% isopropanol or 70% etha

xMAP Technology System StartupPN 89-00002-00-063 Rev. A 5 - 7“Calibration Succeeded” message appears. The settings and any comment you enter are logge

Luminex 100 User Manual Version 1.7 xMAP Technology5 - 8 PN 89-00002-00-063 Rev. A3. Check the box in front of the report type you want. You can check

xMAP Technology System StartupPN 89-00002-00-063 Rev. A 5 - 9Figure 5-11. Luminex Calibration Detail ReportThe calibration detail report is often use

PN 89-00002-00-063 Rev. A iContentsIntroduction 1-1Principle of Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1Bas

Luminex 100 User Manual Version 1.7 xMAP Technology5 - 10 PN 89-00002-00-063 Rev. APrime: Removes air pockets from the fluidic pathways. Use this oper

PN 89-00002-00-063 Rev. A 6 - 1Data AcquisitionThe Luminex 100 analyzer uses Luminex Data Collector software to acquire data. This chapter describes t

Luminex 100 User Manual Version 1.7 xMAP Technology6 - 2 PN 89-00002-00-063 Rev. ASound effects: Enables sounds during system operation.Clear recent f

xMAP Technology Data AcquisitionPN 89-00002-00-063 Rev. A 6 - 3Acquisition tab On this tab you can select the default folder for your session files. I

Luminex 100 User Manual Version 1.7 xMAP Technology6 - 4 PN 89-00002-00-063 Rev. AShow APD Voltages. This is a Field Service option. Do not select thi

xMAP Technology Data AcquisitionPN 89-00002-00-063 Rev. A 6 - 5To use the heater, check Enabled in the Heater area. Use the Set Point control to selec

Luminex 100 User Manual Version 1.7 xMAP Technology6 - 6 PN 89-00002-00-063 Rev. ACreate a Session Using Default Settings To create a session using

xMAP Technology Data AcquisitionPN 89-00002-00-063 Rev. A 6 - 72. Select the template that you want to use for this session and click OK. If the templ

Luminex 100 User Manual Version 1.7 xMAP Technology6 - 8 PN 89-00002-00-063 Rev. AOpen and Add to an Existing Session To open an existing session and

xMAP Technology Data AcquisitionPN 89-00002-00-063 Rev. A 6 - 93. Use the settings shown or modify them, then highlight the first vacant sample.4. Cli

ii PN 89-00002-00-063 Rev. ALuminex 100 User Manual Version 1.7 xMAP TechnologyElectronics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Luminex 100 User Manual Version 1.7 xMAP Technology6 - 10 PN 89-00002-00-063 Rev. A2. Make desired setting changes. See “Session Settings” on page 6-1

xMAP Technology Data AcquisitionPN 89-00002-00-063 Rev. A 6 - 11Open. Opens an existing sessionClose Session. Closes the current session. Prompts you

Luminex 100 User Manual Version 1.7 xMAP Technology6 - 12 PN 89-00002-00-063 Rev. AHelp Contents. Opens the online help.Search for Help On. Opens a se

xMAP Technology Data AcquisitionPN 89-00002-00-063 Rev. A 6 - 13Clear All: Clears the screen of all data and graphs. This function deletes all associ

Luminex 100 User Manual Version 1.7 xMAP Technology6 - 14 PN 89-00002-00-063 Rev. ARight-Click Menu Other commands appear on the right-click menu. To

xMAP Technology Data AcquisitionPN 89-00002-00-063 Rev. A 6 - 15We recommend using at least 100 events per microsphere set. This guarantees that each

Luminex 100 User Manual Version 1.7 xMAP Technology6 - 16 PN 89-00002-00-063 Rev. ABead Set tab On the Bead Set tab you can select and name the approp

xMAP Technology Data AcquisitionPN 89-00002-00-063 Rev. A 6 - 17Histogram The histogram is located in the lower-left corner of the screen. The histogr

Luminex 100 User Manual Version 1.7 xMAP Technology6 - 18 PN 89-00002-00-063 Rev. A2. Click X axis, then click on the desired parameter name.If you se

xMAP Technology Data AcquisitionPN 89-00002-00-063 Rev. A 6 - 19Use the Show Bead options to set the histogram to show events for only one bead set, a

PN 89-00002-00-063 Rev. A iiixMAP Technology ContentsFile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-10Edit

Luminex 100 User Manual Version 1.7 xMAP Technology6 - 20 PN 89-00002-00-063 Rev. Afor later assays or sessions. By increasing this voltage setting, y

xMAP Technology Data AcquisitionPN 89-00002-00-063 Rev. A 6 - 21density is indicated by contrasting colors. A decaying dot plot shows the 100 most rec

Luminex 100 User Manual Version 1.7 xMAP Technology6 - 22 PN 89-00002-00-063 Rev. Athe beads are hitting with respect to their region. The beads shoul

xMAP Technology Data AcquisitionPN 89-00002-00-063 Rev. A 6 - 23The message at the top reflects the current status of the Luminex 100 analyzer.Ready:

Luminex 100 User Manual Version 1.7 xMAP Technology6 - 24 PN 89-00002-00-063 Rev. AThe system monitor section also shows information about temperature

xMAP Technology Data AcquisitionPN 89-00002-00-063 Rev. A 6 - 255. In the results table of the new session, highlight the sample where you want to beg

Luminex 100 User Manual Version 1.7 xMAP Technology6 - 26 PN 89-00002-00-063 Rev. A9. Remove the microtiter plate from the plate holder.10. Click Retr

xMAP Technology Data AcquisitionPN 89-00002-00-063 Rev. A 6 - 279. Remove the microtiter plate from the plate holder.10. Click Retract from the toolba

Luminex 100 User Manual Version 1.7 xMAP Technology6 - 28 PN 89-00002-00-063 Rev. A To interrupt sample acquisition between sample runs:1. Click Canc

xMAP Technology Data AcquisitionPN 89-00002-00-063 Rev. A 6 - 296. In the Manufacturers List, click Generic. In Printers list, click Generic/Text Only

iv PN 89-00002-00-063 Rev. ALuminex 100 User Manual Version 1.7 xMAP TechnologyTroubleshooting 8-1Power Supply Problems . . . . . . . . . . . . . . .

Luminex 100 User Manual Version 1.7 xMAP Technology6 - 30 PN 89-00002-00-063 Rev. A

PN 89-00002-00-063 Rev. A 7 - 1Maintenance and CleaningProper maintenance and cleaning of the Luminex 100 analyzer should be performed in order to pre

Luminex 100 User Manual Version 1.7 xMAP Technology7 - 2 PN 89-00002-00-063 Rev. A3. While you are waiting for the analyzer and optics system to warm

xMAP Technology Maintenance and CleaningPN 89-00002-00-063 Rev. A 7 - 3the waste, remove air from the lines by clicking Prime until no more air shows

Luminex 100 User Manual Version 1.7 xMAP Technology7 - 4 PN 89-00002-00-063 Rev. AClean Sample Probe  Clean the sample probe on a monthly basis, or w

xMAP Technology Maintenance and CleaningPN 89-00002-00-063 Rev. A 7 - 52. On the back of the Luminex 100 analyzer, remove the thumb-screw at the top o

Luminex 100 User Manual Version 1.7 xMAP Technology7 - 6 PN 89-00002-00-063 Rev. A3. Replace the filter and replace the screen.4. Plug in and turn on

xMAP Technology Maintenance and CleaningPN 89-00002-00-063 Rev. A 7 - 7Clean Luminex 100 Analyzer Ventilation Filter Check the Luminex 100 analyzer v

Luminex 100 User Manual Version 1.7 xMAP Technology7 - 8 PN 89-00002-00-063 Rev. AChange the Sheath Filter and Quick-disconnect O-rings Change the sh

xMAP Technology Maintenance and CleaningPN 89-00002-00-063 Rev. A 7 - 9Figure 7-6. Power ModuleAdjust Sample Probe Vertical HeightThe vertical height

PN 89-00002-00-063 Rev. A vxMAP Technology ContentsGlossary A-1Product Numbers B-1Hardware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Luminex 100 User Manual Version 1.7 xMAP Technology7 - 10 PN 89-00002-00-063 Rev. A3. In a 96-well microtiter plate where overall height is no more th

xMAP Technology Maintenance and CleaningPN 89-00002-00-063 Rev. A 7 - 1111. Use a 3/32 inch hexagonal allen wrench to tighten the height adjustment lo

Luminex 100 User Manual Version 1.7 xMAP Technology7 - 12 PN 89-00002-00-063 Rev. ADecontaminating the Luminex 100 Analyzer for Return ShipmentTechnic

Luminex 100 Maintenance Log Month(s): ________________________________________Year:_______________Procedure: To use this lo

PN 89-00002-00-063 Rev. A 8 - 1TroubleshootingTroubleshooting procedures help users isolate, identify, and remedy problems with the Luminex 100 analyz

Luminex 100 User Manual Version 1.7 xMAP Technology8 - 2 PN 89-00002-00-063 Rev. Aoutside of the U.S. and Canada can call us at +1 512-381-4397 betwee

xMAP Technology TroubleshootingPN 89-00002-00-063 Rev. A 8 - 3Pressurization Normal air and sheath pressure readings vary between 6-9 psi while the co

Luminex 100 User Manual Version 1.7 xMAP Technology8 - 4 PN 89-00002-00-063 Rev. AFluid Leaks Fluid leaks can result in poor pressurization and failed

xMAP Technology TroubleshootingPN 89-00002-00-063 Rev. A 8 - 5Sample Probe Problems with the sample probe can lead to fluid leaks and pressurization p