Luminex 100 IS Developer Workbench Guide Version 2.3 User Manual

Luminex 100™ IS DeveloperWorkbench Guide Version 2.3Lum/nex®

Luminex 100 IS Developer Workbench Guide Version 2.3 xMAP Technology4 PN 89-00002-00-084 Rev. Band do not exceed a bead concentration of 2.5 x 106 mic

xMAP Technology General GuidelinesPN 89-00002-00-084 Rev. B 5The volume restrictions that the amount expelled back places on the assay design can be

Luminex 100 IS Developer Workbench Guide Version 2.3 xMAP Technology6 PN 89-00002-00-084 Rev. BGating Determine the gating on the doublet discriminato

xMAP Technology Developer Workbench SoftwarePN 89-00002-00-084 Rev. B 7Developer Workbench SoftwareAccess the Developer Workbench Software through t

Luminex 100 IS Developer Workbench Guide Version 2.3 xMAP Technology8 PN 89-00002-00-084 Rev. B To start only the stand-alone Developer Workbench:1.

xMAP Technology Create New ProductPN 89-00002-00-084 Rev. B 9Create New Product Assay kits include products that are standards, controls, or standar

Luminex 100 IS Developer Workbench Guide Version 2.3 xMAP Technology10 PN 89-00002-00-084 Rev. BFigure 1. Create New Product Dialog Box—Standards2. C

xMAP Technology Create New ProductPN 89-00002-00-084 Rev. B 11the button repeats the process. For example, Protein 1 becomes Protein 11, Protein 2 b

Luminex 100 IS Developer Workbench Guide Version 2.3 xMAP Technology12 PN 89-00002-00-084 Rev. B2. Click New to enter a new product into the system. T

xMAP Technology Create New ProductPN 89-00002-00-084 Rev. B 13Standards and Controls  To create a product that consists of standards and controls:1

© LUMINEX CORPORATION, 2005. All rights reserved. No part of this publication may be reproduced, transmitted, transcribed, or translated into any lang

Luminex 100 IS Developer Workbench Guide Version 2.3 xMAP Technology14 PN 89-00002-00-084 Rev. Beach test is selected when you create the template usi

xMAP Technology Create TemplatePN 89-00002-00-084 Rev. B 15Create Template There are four template types:• Quantitative Assay (Standards or Controls

Luminex 100 IS Developer Workbench Guide Version 2.3 xMAP Technology16 PN 89-00002-00-084 Rev. B2. Type the correct information in the Developing Comp

xMAP Technology Create TemplatePN 89-00002-00-084 Rev. B 17Figure 6. The Product Name column shows all saved products you created. Select the desire

Luminex 100 IS Developer Workbench Guide Version 2.3 xMAP Technology18 PN 89-00002-00-084 Rev. B Figure 7. Select Bead IDs13. In the Min Beads column

xMAP Technology Create TemplatePN 89-00002-00-084 Rev. B 19 Figure 8. Select Curve Fit Formula15. Click Next to display and select commands. See Fi

Luminex 100 IS Developer Workbench Guide Version 2.3 xMAP Technology20 PN 89-00002-00-084 Rev. B17. These commands become part of the template and are

xMAP Technology Create TemplatePN 89-00002-00-084 Rev. B 21Qualitative Assay TemplateYou define each Qualitative template using a single product. Th

Luminex 100 IS Developer Workbench Guide Version 2.3 xMAP Technology22 PN 89-00002-00-084 Rev. B“Quali” value for each test. Control products are opti

xMAP Technology Create TemplatePN 89-00002-00-084 Rev. B 237. Enter the Low Limit and High Limit for the Doublet Discriminator Gate.8. Click Next. T

PN 89-00002-00-084 Rev. B iContentsDeveloper Workbench Guide 1Luminex 100™ IS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1The

Luminex 100 IS Developer Workbench Guide Version 2.3 xMAP Technology24 PN 89-00002-00-084 Rev. BFigure 12. Open Reagent Product Dialog Box—Add a Stan

xMAP Technology Create TemplatePN 89-00002-00-084 Rev. B 25Figure 13. Select Bead IDs13. Under Min Beads, enter the number of beads to collect for

Luminex 100 IS Developer Workbench Guide Version 2.3 xMAP Technology26 PN 89-00002-00-084 Rev. BFigure 14. Select Qualitative FormulaQualitative Form

xMAP Technology Create TemplatePN 89-00002-00-084 Rev. B 27the current values for the selected formula. See Figure 15. You can add a new range, edit

Luminex 100 IS Developer Workbench Guide Version 2.3 xMAP Technology28 PN 89-00002-00-084 Rev. B 15. Click Next to continue with the Select Template C

xMAP Technology Create TemplatePN 89-00002-00-084 Rev. B 29automatically assigned to the next available well. Laboratory personnel can select the st

Luminex 100 IS Developer Workbench Guide Version 2.3 xMAP Technology30 PN 89-00002-00-084 Rev. BFigure 18. Template Setup Wizard—Select Data Collecti

xMAP Technology Create TemplatePN 89-00002-00-084 Rev. B 31Figure 19. Enter the Number of Tests9. Type the number of desired tests in the Tests box

Luminex 100 IS Developer Workbench Guide Version 2.3 xMAP Technology32 PN 89-00002-00-084 Rev. BYou can also select the first bead ID and click Apply

xMAP Technology Create TemplatePN 89-00002-00-084 Rev. B 3315. After you enter all the information into the template, click Save and then click Clos

ii PN 89-00002-00-084 Rev. BLuminex 100 IS Developer Workbench GuideVersion 2.3 xMAP TechnologyCreate Template . . . . . . . . . . . . . . . . . . .

Luminex 100 IS Developer Workbench Guide Version 2.3 xMAP Technology34 PN 89-00002-00-084 Rev. Bincluded in the template name that the lab personnel s

xMAP Technology Template MaintenancePN 89-00002-00-084 Rev. B 35Figure 24. Select Template Commands6. In the Available Commands list, click the des

Luminex 100 IS Developer Workbench Guide Version 2.3 xMAP Technology36 PN 89-00002-00-084 Rev. BYou can print template information directly from the L

xMAP Technology Template MaintenancePN 89-00002-00-084 Rev. B 37Figure 26. Template Setup Wizard—Select Template InformationEdit a UsedTemplateA us

Luminex 100 IS Developer Workbench Guide Version 2.3 xMAP Technology38 PN 89-00002-00-084 Rev. B2. Select the template to edit (the template ID cell d

xMAP Technology Template MaintenancePN 89-00002-00-084 Rev. B 39Figure 29. Open Template Dialog Box—Select Unused Template to Delete2. Select the t

Luminex 100 IS Developer Workbench Guide Version 2.3 xMAP Technology40 PN 89-00002-00-084 Rev. BFigure 30. Open Template Dialog Box—Select Used Templ

xMAP Technology Template MaintenancePN 89-00002-00-084 Rev. B 41.Figure 31. Open Template Dialog Box—Select Template To PrintYou can print a templa

Luminex 100 IS Developer Workbench Guide Version 2.3 xMAP Technology42 PN 89-00002-00-084 Rev. BFigure 33. Template Setup Wizard—Print a Template4. C

xMAP Technology Template MaintenancePN 89-00002-00-084 Rev. B 436. Click Print at the top of the Template Report dialog box. The Windows Print dialo

PN 89-00002-00-084 Rev. B iiixMAP Technology ContentsProcedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57E

Luminex 100 IS Developer Workbench Guide Version 2.3 xMAP Technology44 PN 89-00002-00-084 Rev. B Figure 36. Import Template Dialog BoxExport a Templa

xMAP Technology HelpPN 89-00002-00-084 Rev. B 45Figure 38. Template Saved Dialog Box3. Click OK.4. Copy the template from the folder to a diskette

Luminex 100 IS Developer Workbench Guide Version 2.3 xMAP Technology46 PN 89-00002-00-084 Rev. BSystem Information  To display system information:1.

xMAP Technology HelpPN 89-00002-00-084 Rev. B 47From the opening help screen select the desired topic from Contents in the left pane or follow the t

Luminex 100 IS Developer Workbench Guide Version 2.3 xMAP Technology48 PN 89-00002-00-084 Rev. B

PN 89-00002-00-084 Rev. B 49xMAP ProtocolsUpdates or additions to these protocols are posted on the Luminex Tech Support website at http://luminexcorp

Luminex 100 IS Developer Workbench Guide Version 2.3 xMAP Technology50 PN 89-00002-00-084 Rev. BVortexing and sonicating are not effective in resuspen

PN 89-00002-00-084 Rev. B 51xMAP Technology xMAP Microsphere HandlingIt is not advisable to construct an assay without defining the concentration of

Luminex 100 IS Developer Workbench Guide Version 2.3 xMAP Technology52 PN 89-00002-00-084 Rev. BProtein Coupling ProtocolIntroduction Use these protoc

PN 89-00002-00-084 Rev. B 53xMAP Technology Protein Coupling ProtocolPreparation 1. Allow all reagents to warm to room temperature.2. Using an analyt

iv PN 89-00002-00-084 Rev. BLuminex 100 IS Developer Workbench GuideVersion 2.3 xMAP Technology

Luminex 100 IS Developer Workbench Guide Version 2.3 xMAP Technology54 PN 89-00002-00-084 Rev. BCoupling,Blocking, andStorage1. Add the protein prepar

PN 89-00002-00-084 Rev. B 55xMAP Technology Protein Coupling Protocoldifferent buffer. If your protein does not couple satisfactorily under these rec

Luminex 100 IS Developer Workbench Guide Version 2.3 xMAP Technology56 PN 89-00002-00-084 Rev. BOligonucleotide Coupling ProtocolIntroduction Use thes

PN 89-00002-00-084 Rev. B 57xMAP Technology Oligonucleotide Coupling ProtocolProcedure 1. Disperse the pellet with sonication, and vortex the contain

Luminex 100 IS Developer Workbench Guide Version 2.3 xMAP Technology58 PN 89-00002-00-084 Rev. BEnumerate the Coupled Microspheres1. Dilute the resusp

PN 89-00002-00-084 Rev. B 59xMAP Technology LumAvidin Binding ProtocolLumAvidin Binding ProtocolIntroduction Use these protocols as a general starti

Luminex 100 IS Developer Workbench Guide Version 2.3 xMAP Technology60 PN 89-00002-00-084 Rev. B5. Aspirate the supernatant and add 250 µL of the biot

PN 89-00002-00-084 Rev. B 61Compatibility with Luminex 100 Version 1.7Certain settings in the Luminex 100 Version 1.7 software must have particular va

Luminex 100 IS Developer Workbench Guide Version 2.3 xMAP Technology62 PN 89-00002-00-084 Rev. BFigure 40. Options Dialog Box—User Tab3. Click New Se

PN 89-00002-00-084 Rev. B 63Customize Splash ScreenSplash Screen When the Luminex 100™ IS 2.3 software starts, the Luminex splash screen displays for

PN 89-00002-00-084 Rev. B 1Developer Workbench GuideLuminex 100™ IS The Luminex 100 IS system was developed to increase efficiency in the clinical l

Luminex 100 IS Developer Workbench Guide Version 2.3 xMAP Technology64 PN 89-00002-00-084 Rev. B To enter the path and dimensions for the graphic tha

PN 89-00002-00-084 Rev. B 65AAbout Developer Workbench 45about this guide 1Acquire 20Acquire Background 20Acquire Control 20Acquire Standard 20Acquire

66 PN 89-00002-00-084 Rev. B Luminex IS Developer Workbench Guide Version 2.3 xMAP Technologyformula, volume restrictions 5formulas, algorithm 28form

PN 89-00002-00-084 Rev. B 67xMAP Technology Indextemplate types 15Uuser manual, conventions 1VVacuum 51value settings, Luminex 100 Version 1.7 61Veri

68 PN 89-00002-00-084 Rev. B Luminex IS Developer Workbench Guide Version 2.3 xMAP Technology

Luminex 100 IS Developer Workbench Guide Version 2.3 xMAP Technology2 PN 89-00002-00-084 Rev. BGeneral GuidelinesBead HandlingLimitations • xMAP micro

xMAP Technology General GuidelinesPN 89-00002-00-084 Rev. B 3HandlingInstructionsxMAP Carboxylated Microspheres come in various configurations. The