Luminex xPONENT for MAGPIX User Manual download pdf (Page 19)

Classification and Reporter Fluorochromes

MagPix beads in the calibration kit are used to autofocus the camera and calibrate the CL1,

CL2, and RP1 channels. The beads in the verification kit are a mix of 6 different regions that

cover the range of the 50-plex map. Both calibration and verification beads are triple-dyed,

and the fluorescence signal of these dyes enables classification of each bead set.

TABLE 1.

MAGPIX Active Bead Regions (by Region)

Region Region Region

MC10012 MC10013 MC10014

MC10015 MC10018 MC10019

MC10020 MC10021 MC10022

MC10025 MC10026 MC10027

MC10028 MC10029 MC10030

MC10033 MC10034 MC10035

MC10036 MC10037 MC10038

MC10039 MC10042 MC10043

MC10044 MC10045 MC10046

MC10047 MC10048 MC10051

MC10052 MC10053 MC10054

MC10055 MC10056 MC10057

MC10061 MC10062 MC10063

MC10064 MC10065 MC10066

MC10067 MC10072 MC10073

MC10074 MC10075 MC10076

MC10077 MC10078

Fluidics 1 and Fluidics 2

Although it undergoes a wash step in between wells, the probe can be susceptible to carry-

over from well-to-well. Fluidics 1 contains one bead set. Fluidics 2 contains a buffer solution

and a different control bead. The function of this maintenance procedure is to measure how

much (as a percentage) of the first bead set in Fluidics 1 is found in the well where Fluidics 2

has been loaded.

Sample Volume

Sample volumes range in size from 20 µL to 200 µL. Ensure that approximately 25 µL more

than the sample volume remains in the well after aspiration. This amount may vary

depending on the type of plate used. Your sample volume must be large enough to prevent

aspirating air into the fluid line when acquiring sample, and small enough to prevent spill-over

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Luminex xPONENT for MAGPIX User Manual Page 19