Luminex FLEXMAP 3D User Manual User Manual download pdf (Page 21)

This chapter reviews the technical aspects of the Luminex

FLEXMAP 3D

system:

operation, system components, subsystems, recommended additional equipment, and

specifications.

How the FLEXMAP 3D System Operates

The Luminex

FLEXMAP 3D

system, in combination with xMAP

(Multi-Analyte Profiling)

technology, will simultaneously measure up to 500 analytes from a single sample. xMAP

technology incorporates microspheres, proprietary dyeing processes, flow cytometry based

fluidics, lasers, the latest in high-speed digital signal and advanced computer algorithms,

enabling a multi-analyte detection system that demonstrates excellent sensitivity and

specificity.

An established proprietary process created by Luminex to internally dye same-size

polystyrene microspheres with multiple fluorophores facilitates the creation of five hundred

distinguishable microspheres when these fluorophores are combined in varying

concentrations. The microspheres, discriminated by color, are excited by two lasers in the

FLEXMAP 3D

instrument. The resulting emission is detected by avalanche photo diodes

(APDs) in three classification channels (CL1, CL2, and CL3) that is then further analyzed

using a separate APD in a doublet discriminator (DD) channel, which measures bead size

through side-scatter.

Analytes are bound to xMAP microspheres using the same surface chemistry used with

earlier Luminex instruments (LX100/200). Reporters, tagged with fluorescent labels excited at

a different wavelength than the internal dyes, bind to the analyte of interest and are detected

by a photomultiplier tube (PMT) in a reporter channel (RP1), allowing for quantitative

analysis. As the microspheres in a fluid stream pass rapidly through the laser beams, high-

speed digital signal and computer algorithms discriminate which analyte is being carried on

each microsphere and quantifies the reaction based on fluorescent reporter signal. The

results are analyzed by the system software and presented in a readable format for analysis.

Chapter 2: Technical Overview

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Luminex FLEXMAP 3D User Manual User Manual Page 21