Luminex FLEXMAP 3D User Manual User Manual download pdf (Page 60)

Symptom Possible cause Solution

The microspheres are photobleached. Replace the microspheres with

unphotobleached microspheres.

There are an insufficient number of beads

in the sample.

Ensure that there are 2000 to 5000 beads

per bead set per well.

The sample is too concentrated. Dilute concentrated biological fluids, such

as serum or plasma, at least 1:5.

Bead Detail Irregularities

Use these tools to assist in diagnosing system and kit-related problems:

•

instrument calibrators

• instrument verifiers

• assay standards

• assay controls

• error messages

Review calibration/control trend reports routinely to detect trends.

Use instrument Luminex

xMAP

verification microspheres to check the success of the

instrument calibration and to troubleshoot. If there is a problem with kit results, xMAP verifiers

can help determine if the problem is related to the instrument. If calibration and verification

are successful, contact the kit manufacturer.

The Bead Detail Irregularities table displays irregular groupings of beads. Compare the

displays to the following bead detail of a normal bead grouping - a tight bead population

within a white region.

FIGURE 22.

Normal Bead Detail

TABLE 8.

Verification Problems Continued

Luminex

FLEXMAP 3D

Hardware User Manual

For research use only. Not for use

in diagnostic procedures.

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Luminex FLEXMAP 3D User Manual User Manual Page 60