Luminex FLEXMAP 3D User Manual User Manual download pdf (Page 57)

Symptom Possible cause Solution

The microtiter plate is warped. Inspect the microtiter plate. Replace it if it

is warped.

The sample probe is bent. Remove the sample probe from the

instrument. Roll it on a flat surface. If a

sample probe has been bent and rolled

straight more than once, discard it and

replace it with a new sample probe.

Perform an automatic sample probe height

adjustment using the software.

Calibration Problems

TABLE 6.

Calibration Problems

Symptom Possible cause Solution

Calibration is slow or fails. The calibration microspheres are not fully

suspended.

Vortex the calibration vials to resuspend

the microspheres.

The wrong lot number or target values are

entered in the appropriate software user

interface.

Check the lot number and target values to

make sure they are correct.

The instrument calibrators are in the wrong

well.

Verify that calibration microspheres are in

the correct well.

There are not enough calibrator

microspheres in the well.

Add at least five drops of calibrator

microspheres to the well. For accurate

drop volume, hold the vial upside down at

a 90 degree angle to the microtiter plate

while dispensing them.

The calibrator lot is expired. Use an unexpired bottle of calibrator

microspheres.

The sample probe height is incorrect. Perform an automatic sample probe height

adjustment.

The sample probe is clogged. See the section titled "Clogs".

There is a partial clog in the instrument. See the section titled "Clogs".

There is air in the instrument. The waste

line was moved during instrument

operation, resulting in an unstable flow

rate.

Perform an automatic sample probe height

adjustment. Use the software to run a

prime command three times, an alcohol

flush command twice, then a wash

command three time with deionized water.

The waste line was moved during

instrument operation, resulting in an

unstable flow rate.

Stabilize the waste line during instrument

operation. See the section titled

“Maintaining Fluids”.

TABLE 5.

Sample Probe Problems Continued

For research use only. Not for use in diagnostic procedures.

Troubleshooting

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