Luminex FLEXMAP 3D User Manual User Manual download pdf (Page 55)

Clogs

Often, a clog somewhere in the Luminex

FLEXMAP 3D

instrument is the cause of a fluid

leak, a pressurization or sample probe problem, or a problem with calibration, verification, or

data acquisition.

To determine whether there is a clog, check the bead count during calibration. During

calibration you should see 300 bead events per second or more. If there are fewer than that,

there is probably a clog in the instrument.

In the event you encounter a problem that is clog-related, use the following procedure.

To troubleshoot a possible clog:

Clean and adjust the sample probe. See the section titled “Cleaning the Sample Probe”.

2. Perform the procedure for removing clogs. See the section titled “Removing Clogs”.

3. Replace all fluids.

4. Use the software to run the weekly maintenance routine.

5. Run calibration and verification.

If this procedure is unsuccessful, call Luminex Technical Support.

Pressurization Problems

Normal air and sheath pressure readings vary between 8 and 13 psi while the compressor

runs. If the instrument pressure is out of range, sample acquisition either can fail or can

return inadequate results.

TABLE 3.

Pressurization Problems

Symptom Possible cause Solution

Pressurization fails or pressure is

too low.

The sheath and waste lines are not fully

connected.

Check the lines between the sheath and

waste bottles and the

Luminex

FLEXMAP

instrument.

The sheath or waste fittings are cracked. Inspect the fittings to be sure they form a

tight seal. If not, call Technical Support.

There is a leak in the instrument. Check for leaks. A leak is obvious if there

is fluid on the surface where the FLEXMAP

3D instrument sits. See the section titled

“Fluid Leaks”.

The compressor does not engage. Use the software to run a prime command.

If you do not hear the compressor turn on,

contact Technical Support.

For research use only. Not for use in diagnostic procedures.

Troubleshooting

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