Luminex FLEXMAP 3D User Manual User Manual Page 58

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Symptom Possible cause Solution
There is a problem internal to the
instrument.
Review the log of calibration reports.
Check for dramatic changes in
temperature, sheath pressure, or voltage. If
any of these are present, contact Technical
Support.
No events are collected during
calibration.
There is a problem with fluid levels. Check the sheath and waste fluid levels.
Verify that tubing for both bottles is tightly
connected to the instrument. Check that
the waste fluid container cap is vented.
The sample probe is clogged. See the section titled "Clogs".
The Cheminert fitting is loose. Tighten the Cheminert fitting.
Verification Problems
TABLE 7.
Verification Problems
Symptom Possible cause Solution
The instrument fails verification. The verification microspheres are not fully
suspended.
Vortex the verification vials to resuspend
the microspheres.
The wrong lot number or target values are
entered in the appropriate page of the
software UI.
Check the lot number and target values to
make sure they are correct.
The instrument verification microspheres
are in the wrong well.
Verify that verification microspheres are in
the correct well.
There are not enough verification
microspheres in the well.
Add at least five drops of verification
microspheres to the well. For accurate
drop volume, hold the vial upside down at
a 90 degree angle to the microtiter plate
while dispensing them.
The verification lot is expired. Use an unexpired bottle of verification
microspheres.
The verification microspheres have been
diluted.
Do not dilute the verification microspheres.
The sample probe height is incorrect. Perform an automatic sample probe height
adjustment.
The sample probe is clogged. See the section titled “Clogs”.
There is air in the instrument. Verify the sample probe height. Use the
software to run a prime command three
times, an alcohol flush command twice,
then a wash command three times with
deionized water.
TABLE 6.
Calibration Problems Continued
Luminex
®
FLEXMAP 3D
®
Hardware User Manual
48
For research use only. Not for use
in diagnostic procedures.
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